Abstract
We developed a molecular beacon targeting miR-141-3p, aberrantly increased in 5-fluorouracil-resistant colorectal cancer cells (R-CRCCs). It consists of a fluorophore-labeled oligonucleotide, antisense to miR-141-3p, and a quencher. It detected R-CRCCs and recovered the chemosensitivity of them to 5-fluorouracil by hybridization with miR-141-3p, which is applicable to cancer treatment.
MeSH terms
-
Antagomirs / metabolism
-
Apoptosis / drug effects*
-
Cell Line, Tumor
-
Colorectal Neoplasms / metabolism
-
Colorectal Neoplasms / pathology
-
DNA-Binding Proteins / chemistry
-
DNA-Binding Proteins / genetics
-
DNA-Binding Proteins / metabolism*
-
Drug Resistance, Neoplasm / drug effects
-
Fluorescence Recovery After Photobleaching
-
Fluorouracil / pharmacology*
-
Humans
-
MicroRNAs / antagonists & inhibitors
-
MicroRNAs / genetics
-
MicroRNAs / metabolism*
-
Microscopy, Confocal
-
Nucleic Acid Hybridization
-
Transforming Growth Factor beta / metabolism
-
Tumor Suppressor Proteins / chemistry
-
Tumor Suppressor Proteins / genetics
-
Tumor Suppressor Proteins / metabolism*
Substances
-
Antagomirs
-
DNA-Binding Proteins
-
MIRN141 microRNA, human
-
MicroRNAs
-
TRIM13 protein, human
-
Transforming Growth Factor beta
-
Tumor Suppressor Proteins
-
Fluorouracil