Characterization of hemicelluloses in Phyllostachys edulis (moso bamboo) culm during xylogenesis

Kai-Li Wang; Bo Wang; Ruibo Hu; Xianhai Zhao; Huiling Li; Gongke Zhou; Lili Song; Ai-Min Wu

doi:10.1016/j.carbpol.2019.05.088

Characterization of hemicelluloses in Phyllostachys edulis (moso bamboo) culm during xylogenesis

Carbohydr Polym. 2019 Oct 1:221:127-136. doi: 10.1016/j.carbpol.2019.05.088. Epub 2019 May 30.

Authors

Kai-Li Wang¹, Bo Wang², Ruibo Hu³, Xianhai Zhao⁴, Huiling Li¹, Gongke Zhou³, Lili Song⁵, Ai-Min Wu⁶

Affiliations

¹ State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangzhou, 510642, China; Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architectures, South China Agricultural University, Guangzhou, 510642, China.
² The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou, 510642, Guangdong, China.
³ College of Resource and Environment, Qingdao Agricultural University, Qingdao, 266109, China.
⁴ Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architectures, South China Agricultural University, Guangzhou, 510642, China; Institute of Plant and Food Sciences, Department of Biology, Southern University of Science and Technology, Shenzhen, 518055, China.
⁵ State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin'an, 311300, Zhejiang, China. Electronic address: [email protected].
⁶ State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangzhou, 510642, China; Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architectures, South China Agricultural University, Guangzhou, 510642, China. Electronic address: [email protected].

PMID: 31227151
DOI: 10.1016/j.carbpol.2019.05.088

Abstract

Hemicelluloses are β-(1→4)-linked backbone polysaccharides found in plant cell walls that include xyloglucans, xylans, mannans and glucomannans, and play important roles in plant tissue configuration. In this study, hemicelluloses were isolated from the apical, middle and basal segments of 6 m Phyllostachys edulis culm using KOH and DMSO extraction procedures, respectively. Chemical composition and structural characterization of hemicellulosic fractions were comparatively investigated by a combination of HPLC, GPC, FT-IR, ¹H-, ¹³C-, HSQC NMR and TGA techniques. Our results show that the main chain of hemicellulose in P. edulis consists of glucuronoarabinoxylans (GAXs) with backbone 1, 4-β-d-Xyl, and side chain arabinose, glucuronic acid and acetylation. Hemicellulose content and molecular weight increased with culm xylogenesis in P. edulis. Our results provide new insights on the dynamics of hemicellulose structure in culm xylogenesis in P. edulis.

Keywords: Arabinose; Hemicellulose; Phyllostachys edulis; Structural characterization.

MeSH terms

Acetylation
Molecular Weight
Polysaccharides / chemistry
Polysaccharides / metabolism*
Sasa / chemistry
Sasa / metabolism*
Xylans / chemistry
Xylans / metabolism
Xylem / cytology
Xylem / metabolism*

Substances

Polysaccharides
Xylans
hemicellulose