Comprehensive and quantitative analysis of G1 cyclins. A tool for studying the cell cycle

PLoS One. 2019 Jun 25;14(6):e0218531. doi: 10.1371/journal.pone.0218531. eCollection 2019.

Abstract

In eukaryotes, the cell cycle is driven by the actions of several cyclin dependent kinases (CDKs) and an array of regulatory proteins called cyclins, due to the cyclical expression patterns of the latter. In yeast, the accepted pattern of cyclin waves is based on qualitative studies performed by different laboratories using different strain backgrounds, different growing conditions and media, and different kinds of genetic manipulation. Additionally, only the subset of cyclins regulating Cdc28 was included, while the Pho85 cyclins were excluded. We describe a comprehensive, quantitative and accurate blueprint of G1 cyclins in the yeast Saccharomyces cerevisiae that, in addition to validating previous conclusions, yields new findings and establishes an accurate G1 cyclin blueprint. For the purposes of this research, we produced a collection of strains with all G1 cyclins identically tagged using the same and most respectful procedure possible. We report the contribution of each G1 cyclin for a broad array of growing and stress conditions, describe an unknown role for Pcl2 in heat-stress conditions and demonstrate the importance of maintaining the 3'UTR sequence of cyclins untouched during the tagging process.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Cycle
  • Cyclin G1 / classification
  • Cyclin G1 / genetics*
  • Cyclin G1 / metabolism
  • Genotype
  • Saccharomyces cerevisiae / cytology
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins / classification
  • Saccharomyces cerevisiae Proteins / genetics*
  • Saccharomyces cerevisiae Proteins / metabolism
  • Stress, Physiological

Substances

  • Cyclin G1
  • Saccharomyces cerevisiae Proteins

Grants and funding

RC was awarded with a Generalitat de Catalunya grant. JC received a grant from the Spanish Government (MINECO Grant Ref: BFU 2013-44189-P and PGC2018-096597-B-I00). JJ received a grant from the Spanish Government (PGC2018-096597-B-I00). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.