Immune responses upon in ovo HVT-IBD vaccination vary between different chicken lines

Dev Comp Immunol. 2019 Nov:100:103422. doi: 10.1016/j.dci.2019.103422. Epub 2019 Jun 24.

Abstract

The genotype of chickens is assumed to be associated with variable immune responses. In this study a modern, moderate performing dual-purpose chicken line (DT) was compared with a high-performing layer-type (LT) as well as a broiler-type (BT) chicken line. One group of each genotype was vaccinated in ovo with a recombinant herpesvirus of turkeys expressing the virus protein VP2 of the infectious bursal disease virus (HVT-IBD) while one group of each genotype was left HVT-IBD unvaccinated (control group). Genotype associated differences in innate and adapted immune responses between the groups were determined over five weeks post hatch. HVT-IBD vaccination significantly enhanced humoral immune responses against subsequently applied live vaccines compared to non-HVT-IBD vaccinated groups at some of the investigated time points (P < 0.05). In addition HVT-IBD vaccination had depending on the genotype a significant impact on splenic macrophage as well as bursal CD4+ T-cell numbers (P < 0.05). On the other hand, the detectable genotype influence on Interferon (IFN) γ and nitric oxide (NO) release of ex vivo stimulated spleen cells was independent of HVT-IBD vaccination. The results of our study suggest considering a genotype specific vaccination regime in the field.

Keywords: Acquired immunity; Chicken genotype; Development; Dual-purpose chicken; HVT-Recombinant; Innate immunity; Vaccination.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animal Husbandry / methods
  • Animals
  • Birnaviridae Infections / immunology
  • Birnaviridae Infections / prevention & control*
  • Birnaviridae Infections / virology
  • Breeding
  • Chick Embryo / growth & development
  • Chick Embryo / immunology
  • Chickens / genetics
  • Chickens / immunology*
  • Chickens / virology
  • Genotype
  • Immunity, Humoral / genetics*
  • Immunogenicity, Vaccine
  • Infectious bursal disease virus / genetics
  • Infectious bursal disease virus / immunology*
  • Specific Pathogen-Free Organisms
  • Turkeys / virology
  • Vaccination / methods
  • Vaccines, Attenuated / administration & dosage
  • Vaccines, Attenuated / immunology
  • Vaccines, Synthetic / administration & dosage
  • Vaccines, Synthetic / immunology
  • Viral Structural Proteins / genetics
  • Viral Structural Proteins / immunology
  • Viral Vaccines / administration & dosage
  • Viral Vaccines / immunology*

Substances

  • VP2 protein, infectious bursal disease virus
  • Vaccines, Attenuated
  • Vaccines, Synthetic
  • Viral Structural Proteins
  • Viral Vaccines