Compositional analysis and biological characterization of Cornus officinalis on human 1.1B4 pancreatic β cells

Mol Cell Endocrinol. 2019 Aug 20:494:110491. doi: 10.1016/j.mce.2019.110491. Epub 2019 Jun 27.

Abstract

Type 1 diabetes (T1D) is an autoimmune disease resulting from the loss of pancreatic β cells and subsequent insulin production. Novel interventional therapies are urgently needed that can protect existing β cells from cytokine-induced death and enhance their function before symptomatic onset. Our initial evidence is suggesting that bioactive ingredients within Cornus officinalis (CO) may be able to serve in this function. CO has been extensively used in Traditional Chinese Medicine (TCM) and reported to possess both anti-inflammatory and pro-metabolic effects. We hypothesize that CO treatment may provide a future potential candidate for interventional therapy for early stage T1D prior to significant β cell loss. Our data demonstrated that CO can inhibit cytokine-mediated β cell death, increase cell viability and oxidative capacity, and increase expression of NFATC2 (Nuclear Factor of Activated T Cells, Cytoplasmic 2). We have also profiled the bioactive components in CO from multiple sources by HPLC/MS (High Performance Liquid Chromatography/Mass Spectrometry) analysis. Altogether, CO significantly increases the energy metabolism of β cells while inducing the NFAT pathway to signal for increased proliferation and endocrine function.

Keywords: Cornus offinicalis; NFAT; Pancreatic beta cells; Traditional Chinese medicine; Type 1 diabetes.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Cell Line
  • Cell Proliferation / drug effects
  • Cell Respiration / drug effects
  • Cell Survival / drug effects
  • Cornus / chemistry*
  • Cytokines / pharmacology
  • Glycolysis / drug effects
  • Humans
  • Insulin-Secreting Cells / drug effects
  • Insulin-Secreting Cells / metabolism*
  • Mitochondria / drug effects
  • Mitochondria / metabolism
  • NFATC Transcription Factors / metabolism
  • Phenotype
  • Phytochemicals / chemistry
  • Phytochemicals / pharmacology
  • Plant Extracts / chemistry*
  • Plant Extracts / pharmacology*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Th1 Cells / drug effects
  • Time Factors
  • Transcriptome / genetics
  • Up-Regulation / drug effects

Substances

  • Cytokines
  • NFATC Transcription Factors
  • Phytochemicals
  • Plant Extracts
  • RNA, Messenger