Plasma FGF-21 and GDF-15 are elevated in different inherited metabolic diseases and are not diagnostic for mitochondrial disorders

J Inherit Metab Dis. 2019 Sep;42(5):918-933. doi: 10.1002/jimd.12142. Epub 2019 Jul 24.

Abstract

Recently, the plasma cytokines FGF-21 and GDF-15 were described as cellular metabolic regulators. They share an endocrine function and are highly expressed in the liver under stress and during starvation. Several studies found that these markers have high sensitivity and specificity for the diagnosis of mitochondrial diseases, especially those with prominent muscular involvement. In our study, we aimed to determine whether these markers could help distinguish mitochondrial diseases from other groups of inherited diseases. We measured plasma FGF-21 and GDF-15 concentrations in 122 patients with genetically confirmed primary mitochondrial disease and 127 patients with non-mitochondrial inherited diseases. Although GDF-15 showed better analytical characteristics (sensitivity = 0.66, specificity = 0.64, area under the curve [AUC] = 0.88) compared to FGF-21 (sensitivity = 0.51, specificity = 0.76, AUC = 0.78) in the pediatric group of mitochondrial diseases, both markers were also elevated in a variety of non-mitochondrial diseases, especially those with liver involvement (Gaucher disease, galactosemia, glycogenosis types 1a, 1b, 9), organic acidurias and some leukodystrophies. Thus, the overall positive and negative predictive values were not acceptable for these measurements to be used as diagnostic tests for mitochondrial diseases (FGF-21 positive predictive value [PPV] = 34%, negative predictive value [NPV] = 73%; GDF-15 PPV = 47%, NPV = 28%). We suggest that FGF-21 and GDF-15 increase in patients with metabolic diseases with metabolic or oxidative stress and inflammation.

Keywords: FGF-21 cytokine; GDF-15 cytokine; circulating plasma cytokines; laboratory diagnostics; mitochondrial diseases; mitochondrial diseases biomarkers.

MeSH terms

  • Adolescent
  • Adult
  • Biomarkers / blood
  • Case-Control Studies
  • Child
  • Child, Preschool
  • Female
  • Fibroblast Growth Factors / blood*
  • Growth Differentiation Factor 15 / blood*
  • Humans
  • Infant
  • Male
  • Metabolic Diseases / blood*
  • Metabolic Diseases / diagnosis*
  • Middle Aged
  • Mitochondrial Diseases / blood
  • Mitochondrial Diseases / diagnosis
  • Predictive Value of Tests
  • Young Adult

Substances

  • Biomarkers
  • Growth Differentiation Factor 15
  • fibroblast growth factor 21
  • Fibroblast Growth Factors