The 35S promoter-driven mDII auxin control sensor is uniformly distributed in leaf primordia

Chunmei Guan; Fei Du; Yuanyuan Xiong; Yuling Jiao

doi:10.1111/jipb.12853

The 35S promoter-driven mDII auxin control sensor is uniformly distributed in leaf primordia

J Integr Plant Biol. 2019 Nov;61(11):1114-1120. doi: 10.1111/jipb.12853. Epub 2019 Sep 11.

Authors

Chunmei Guan¹, Fei Du¹, Yuanyuan Xiong^{1

2}, Yuling Jiao^{1

2}

Affiliations

¹ State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
² College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China.

PMID: 31267663
DOI: 10.1111/jipb.12853

Abstract

By using mechanical and optical sectioning of DII/mDII and R2D2 auxin sensors, we reconfirmed the presence of asymmetric auxin signaling in leaf primordia. We also showed that the imaging data reported by Bhatia et al. (2019) may suffer from artefacts, and that their analysis was artificially biased due to an arbitrary domain demarcation.

MeSH terms

Arabidopsis / genetics*
Arabidopsis Proteins / metabolism*
DNA-Binding Proteins / metabolism
Gene Expression Regulation, Plant
Indoleacetic Acids / metabolism*
Microscopy, Confocal
Plant Growth Regulators / genetics*
Plant Growth Regulators / metabolism
Plant Leaves / growth & development
Plant Leaves / metabolism
Plants, Genetically Modified / metabolism
Promoter Regions, Genetic
Signal Transduction
Transcription Factors / metabolism

Substances

Arabidopsis Proteins
DNA-Binding Proteins
Indoleacetic Acids
MONOPTEROS protein, Arabidopsis
Plant Growth Regulators
Transcription Factors