Lymphokine-induced MHC class II antigen expression on cultured retinal pigment epithelial cells and the influence of cyclosporin A

J Liversidge; H F Sewell; A W Thomson; J V Forrester

Lymphokine-induced MHC class II antigen expression on cultured retinal pigment epithelial cells and the influence of cyclosporin A

Immunology. 1988 Feb;63(2):313-7.

Authors

J Liversidge¹, H F Sewell, A W Thomson, J V Forrester

Affiliation

¹ Department of Ophthalmology, University of Aberdeen, U.K.

PMID: 3127329
PMCID: PMC1454508

Abstract

Culture of rat or guinea-pig retinal pigment epithelium (RPE) cells with Con A-induced lymphokine derived from splenic lymphocytes or rat recombinant IFN-gamma induces strong MHC class II (Ia) antigen expression on up to 40% of RPE cells. Cyclosporin A, which inhibits intravitreal inflammation and Ia antigen expression on RPE cells in vivo, despite a choroidal T-cell infiltrate, suppresses production of Ia-inducing lymphokine but does not suppress in vitro responses of RPE cells to preformed lymphokine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cells, Cultured
Concanavalin A / pharmacology
Cyclosporins / pharmacology*
Dose-Response Relationship, Drug
Guinea Pigs
Histocompatibility Antigens Class II / immunology*
Interferon-gamma / pharmacology
Lymphokines / pharmacology*
Pigment Epithelium of Eye / drug effects
Pigment Epithelium of Eye / immunology*
Rats
Rats, Inbred Strains

Substances

Cyclosporins
Histocompatibility Antigens Class II
Lymphokines
Concanavalin A
Interferon-gamma