Comparison between prochymosin and pepsinogen from lamb and calf

Comp Biochem Physiol B. 1988;89(2):385-91. doi: 10.1016/0305-0491(88)90241-6.

Abstract

1. Prochymosin (EC 3.4.23.4) and pepsinogen A (EC 3.4.23.1) from Mongolian lamb (Ovis platyurea) were purified to homogeneity by salt precipitation, gel filtration and ion-exchange chromatography. 2. Immunoelectrophoresis shows partial immunochemical identity between chymosins and pepsins from lamb and cattle, respectively. 2. Activity determinations, N-terminal amino acid sequences and amino acid compositions also show a close relationship between the proteinases from lamb and cattle. 4. Lamb prochymosin and pepsinogen are both glycosylated.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cattle
  • Chymosin / metabolism*
  • Enzyme Precursors / metabolism*
  • Glycosylation
  • Immunoelectrophoresis
  • Molecular Sequence Data
  • Pepsinogens / metabolism*
  • Sheep
  • Swine

Substances

  • Enzyme Precursors
  • Pepsinogens
  • prorennin
  • Chymosin