A marine bacterial enzymatic cascade degrades the algal polysaccharide ulvan

Nat Chem Biol. 2019 Aug;15(8):803-812. doi: 10.1038/s41589-019-0311-9. Epub 2019 Jul 8.

Abstract

Marine seaweeds increasingly grow into extensive algal blooms, which are detrimental to coastal ecosystems, tourism and aquaculture. However, algal biomass is also emerging as a sustainable raw material for the bioeconomy. The potential exploitation of algae is hindered by our limited knowledge of the microbial pathways-and hence the distinct biochemical functions of the enzymes involved-that convert algal polysaccharides into oligo- and monosaccharides. Understanding these processes would be essential, however, for applications such as the fermentation of algal biomass into bioethanol or other value-added compounds. Here, we describe the metabolic pathway that enables the marine flavobacterium Formosa agariphila to degrade ulvan, the main cell wall polysaccharide of bloom-forming Ulva species. The pathway involves 12 biochemically characterized carbohydrate-active enzymes, including two polysaccharide lyases, three sulfatases and seven glycoside hydrolases that sequentially break down ulvan into fermentable monosaccharides. This way, the enzymes turn a previously unexploited renewable into a valuable and ecologically sustainable bioresource.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins
  • Carbohydrate Metabolism
  • Flavobacteriaceae / enzymology*
  • Gene Expression Regulation, Bacterial
  • Gene Expression Regulation, Enzymologic
  • Genome, Bacterial
  • Genomics
  • Models, Molecular
  • Polysaccharides / chemistry
  • Polysaccharides / metabolism*
  • Protein Conformation
  • Sulfatases / chemistry
  • Sulfatases / genetics
  • Sulfatases / metabolism

Substances

  • Bacterial Proteins
  • Polysaccharides
  • ulvan
  • Sulfatases