Abstract
New strategies are required for the discovery of unknown bioactive molecules produced by gut microbiota in the human host. Herein, we utilize a chemoselective probe immobilized to magnetic beads for analysis of carbonyls in human fecal samples. We identified 112 metabolites due to femtomole analysis and an increased mass spectrometric sensitivity by up to six orders of magnitude.
MeSH terms
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Aldehydes / analysis*
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Aldehydes / metabolism
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Feces / chemistry*
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Feces / microbiology
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Female
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Gastrointestinal Microbiome / physiology*
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Humans
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Ketones / analysis*
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Ketones / metabolism
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Male
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Molecular Probes / analysis*
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Molecular Probes / chemical synthesis
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Molecular Probes / chemistry*
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Molecular Structure
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Particle Size
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Surface Properties
Substances
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Aldehydes
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Ketones
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Molecular Probes