To investigate the effect of chrysin on apoptosis of oral squamous carcinoma KB cell line and the possible mechanisms, and to provide new ideas for the treatment of oral cancer. Methods: Oral cancer KB cells were treated with different concentrations of chrysin (1, 2, 4, 8, 16, and 32 μmol/L) for 24 h. Cell proliferation was detected by MMT assay; apoptosis was detected by flow cytometry; the activity of caspase-3/7 was detected by chemiluminescent assay; mitochondrial membrane potential in KB cells was determined by JC-1 assay; and Western blotting was used to determine the activation of protein kinase B (AKT) and phosphoinositide-3-kinase (PI3K). Results: Chrysin inhibited the proliferation of KB cells in a concentration-dependent manner, accompanied by increase in apoptosis of KB cells, activation of caspase-3/7, decrease in mitochondrial membrane potential, and suppression of the phosphorylation of AKT and PI3K. Conclusion: The effect of chrysin on KB cell apoptosis may be related to mitochondrial dysfunction and inhibition of PI3K/AKT pathway.
目的：探讨白杨素对口腔鳞状细胞癌KB细胞凋亡的影响及其机制，为临床上口腔鳞状细胞癌的治疗提供思路。方法：用不同浓度白杨素(1，2，4，8，16和32 μmol/L)处理KB细胞24 h，采用MMT法检测细胞增殖，流式细胞术检测细胞凋亡，化学发光法检测caspase-3/7活性，JC-1法检测KB细胞线粒体膜电位的变化，蛋白质印迹检测蛋白激酶B(protein kinase B，AKT)和磷脂酰肌醇3-激酶(phosphoinositide-3-kinase，PI3K)的活化。结果：白杨素以浓度依赖方式抑制KB细胞增殖并诱导其凋亡，促进caspase-3/7的活化，降低KB细胞线粒体膜电位；同时抑制AKT和PI3K磷酸化。结论：白杨素诱导KB细胞凋亡作用可能与线粒体功能障碍和抑制PI3K/AKT通路相关。.