Cited2 regulates proliferation and survival in young and old mouse cardiac stem cells

Qiong Wu; Qin Liu; Jinxi Zhan; Qian Wang; Daxiu Zhang; Shuangli He; Shiming Pu; Zuping Zhou

doi:10.1186/s12860-019-0207-2

Cited2 regulates proliferation and survival in young and old mouse cardiac stem cells

BMC Mol Cell Biol. 2019 Jul 17;20(1):25. doi: 10.1186/s12860-019-0207-2.

Authors

Qiong Wu^{1

2

3}, Qin Liu^{1

2

3}, Jinxi Zhan^{1

2

3}, Qian Wang^{1

2

3}, Daxiu Zhang^{1

2

3}, Shuangli He^{1

2

3}, Shiming Pu^{1

2

3}, Zuping Zhou^{4

5

6}

Affiliations

¹ School of Life Sciences, Guangxi Normal University, Guilin, 541004, China.
² Guangxi Universities Key Laboratory of Stem cell and Biopharmaceutical Technology, Guangxi Normal University, Guilin, 541004, China.
³ Research Center for Biomedical Sciences, Guangxi Normal University, Guilin, 541004, China.
⁴ School of Life Sciences, Guangxi Normal University, Guilin, 541004, China. [email protected].
⁵ Guangxi Universities Key Laboratory of Stem cell and Biopharmaceutical Technology, Guangxi Normal University, Guilin, 541004, China. [email protected].
⁶ Research Center for Biomedical Sciences, Guangxi Normal University, Guilin, 541004, China. [email protected].

Abstract

Background: Cardiac stem cells (CSCs) exhibit age-dependent characteristics. Cited2 has been implicated in the regulation of heart development; however, there is little known about how Cited2 affects CSC aging.

Results: Cited2 mRNA and protein level was downregulated in aging heart tissue and CSCs. Old (O)-CSCs showed decreased differentiation and proliferation capacities as compared to Young (Y)-CSCs, the decrease in cell proliferation, increase in apoptosis, and cell cycle arrest in G0/G1 phase in CSCs are mediated by knocdown CITED2expression in (Y)-CSCs.

Conclusions: Cited2 plays an important role in cell cycle progression and in maintaining the balance between CSC proliferation and apoptosis in the process of aging without influencing cell fate decisions. These findings have important implications for cell-based therapies for heart repair.

Keywords: Aging; Apoptosis; Cardiac stem cells; Cited2; Proliferation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Apoptosis / physiology
Cell Differentiation
Cell Proliferation / physiology*
Cell Survival / physiology*
Cell- and Tissue-Based Therapy
Cellular Senescence / physiology*
G1 Phase Cell Cycle Checkpoints
Gene Expression Regulation, Developmental
Gene Knockdown Techniques
Mice
Mice, Inbred C57BL
Myoblasts, Cardiac / cytology
Myoblasts, Cardiac / physiology*
RNA, Small Interfering / genetics
Repressor Proteins / genetics*
Repressor Proteins / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Trans-Activators / genetics*
Trans-Activators / metabolism*
Transcriptome
Transfection

Substances

Cited2 protein, mouse
RNA, Small Interfering
Repressor Proteins
Trans-Activators