Objective: To investigate the proteins expression difference after upregulation of human CD99 in Hodgkin Lymphoma cell line, L428 cell, and verify the function of differential proteins. Methods: The differential proteins were detected by two-dimensional fluorescence difference gel electrophoresis and mass spectrometry analysis, cluster analysis was done by GOfact. Results: There were 38 proteins screened out, of which 21 proteins were positively associated with CD99, while 17 proteins were negative. Among the 38 proteins, 32 proteins participated in biological process, and 35 proteins were involved in the composition and construction. And 28 proteins participated in multifaceted biological activities including antioxidation, protein binding, catalytic activity, regulation of enzyme, signal transduction, molecular structure, regulation of translation and ion transport. Conclusions: The changes of the differential proteins, correlated with cytoskeleton, cell differentiation, signal pathway and regulating gene expression, are closely relevant to the translation between Hodgkin/Reed-Sternberg and B lymphocyte cell.
目的： 探索稳定过表达CD99前后霍奇金淋巴瘤细胞株L428细胞中差异表达的蛋白，并分析验证差异蛋白的功能。 方法： 采用荧光差异凝胶双向电泳和质谱分析技术检测L428细胞稳定过表达CD99前后蛋白表达的差异，采用GOfact开放软件对差异蛋白进行聚类分析，筛选在细胞转化过程中起重要作用的蛋白，并分析验证其功能。 结果： 筛选鉴定得到38个差异蛋白，其中在稳定过表达CD99的霍奇金淋巴瘤细胞株L428（L428-CD99细胞）中与CD99表达呈正相关的蛋白21个，呈负相关的蛋白17个，所得的38个差异蛋白中有32个蛋白参与了细胞的生物过程，35个蛋白参与了细胞的组成和构建，28个参与了抗氧化、蛋白结合、催化活性、酶调节、信号转导、结构分子、翻译调节和离子转运等多个方面。 结论： 筛选得到38个差异蛋白，其中关于细胞骨架、细胞分化、信号通路、调节基因表达等蛋白的改变与H/RS细胞和B淋巴瘤细胞转化相关。.
Keywords: CD99; Hodgkin Lymphoma; Hodgkin/Reed-Sternberg cell; L428 cell; Mass spectrometry analysis.