Attenuated MuV-S79 as vector stably expressing foreign gene

World J Pediatr. 2019 Oct;15(5):511-515. doi: 10.1007/s12519-019-00287-7. Epub 2019 Aug 3.

Abstract

Background: To describe mumps virus (MuV) used as a vector to express enhanced green fluorescent protein (EGFP) or red fluorescent protein (RFP) genes.

Methods: Molecular cloning technique was applied to establish the cDNA clones of recombinant mumps viruses (rMuVs). rMuVs were recovered based on our reverse genetic system of MuV-S79. The properties of rMuVs were determined by growth curve, plaque assay, fluorescent microscopy and determination of fluorescent intensity.

Results: Three recombinant viruses replicated well in Vero cells and similarly as parental rMuV-S79, expressed heterologous genes in high levels, and were genetically stable in at least 15 passages.

Conclusion: rMuV-S79 is a promising platform to accommodate foreign genes like marker genes, other antigens and immunomodulators for addressing various diseases.

Keywords: Foreign genes; Mumps virus; Reverse genetic system.

MeSH terms

  • Animals
  • Chlorocebus aethiops
  • Cloning, Molecular
  • Green Fluorescent Proteins
  • Luminescent Proteins
  • Mumps virus / genetics*
  • Red Fluorescent Protein
  • Reverse Genetics*
  • Vero Cells

Substances

  • Luminescent Proteins
  • enhanced green fluorescent protein
  • Green Fluorescent Proteins

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