Cyclooxygenase inhibitors impair CD4 T cell immunity and exacerbate Mycobacterium tuberculosis infection in aerosol-challenged mice

Commun Biol. 2019 Aug 5:2:288. doi: 10.1038/s42003-019-0530-3. eCollection 2019.

Abstract

Tuberculosis, caused by infection with Mycobacterium tuberculosis (Mtb), kills over 1.6 million people each year despite availability of antibiotics. The increase in drug resistant Mtb strains is a major public health emergency and host-directed therapy as adjunct to antibiotic treatment has gained increased interest. Cyclooxygenase inhibitors (COXi) are frequently used drugs to alleviate tuberculosis related symptoms. Mouse studies of acute intravenous Mtb infection have suggested a potential benefit of COXi for host-directed therapy. Here we show that COXi treatment (ibuprofen and celecoxib) is detrimental to Mtb control in different mouse models of respiratory infection. This effect links to impairments of the Type-1 helper (Th1) T-cell response as CD4 T-cells in COXi-treated animals have significantly decreased Th1 differentiation, reduced IFNγ expression and decreased protective capacity upon adoptive transfer. If confirmed in clinical trials, these findings could have major impact on global health and question the use of COXi for host-directed therapy.

Keywords: Bacteria; Tuberculosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adoptive Transfer
  • Aerosols
  • Animals
  • Bacterial Load
  • Celecoxib / toxicity*
  • Cell Differentiation / drug effects
  • Cyclooxygenase 2 Inhibitors / toxicity
  • Cyclooxygenase Inhibitors / toxicity*
  • Disease Models, Animal
  • Disease Progression
  • Female
  • Host-Pathogen Interactions
  • Ibuprofen / toxicity*
  • Inhalation Exposure
  • Interferon-gamma / immunology
  • Lung / drug effects*
  • Lung / immunology
  • Lung / microbiology
  • Lymphocyte Activation / drug effects
  • Mice, Inbred C3H
  • Mycobacterium tuberculosis / immunology
  • Mycobacterium tuberculosis / pathogenicity*
  • Th1 Cells / drug effects*
  • Th1 Cells / immunology
  • Th1 Cells / microbiology
  • Th1 Cells / transplantation
  • Tuberculosis, Pulmonary / immunology
  • Tuberculosis, Pulmonary / microbiology*

Substances

  • Aerosols
  • Cyclooxygenase 2 Inhibitors
  • Cyclooxygenase Inhibitors
  • IFNG protein, human
  • Interferon-gamma
  • Celecoxib
  • Ibuprofen