Reprogramming of Human Peripheral Blood Mononuclear Cell (PBMC) from a patient suffering of a Werner syndrome resulting in iPSC line (REGUi003-A) maintaining a short telomere length

Stem Cell Res. 2019 Aug:39:101515. doi: 10.1016/j.scr.2019.101515. Epub 2019 Jul 27.

Abstract

Werner syndrome (WS) is a rare human autosomal recessive disorder characterized by early onset of aging-associated diseases, chromosomal instability, and cancer predisposition, without therapeutic treatment solution. Major clinical symptoms of WS include common age-associated diseases, such as insulin-resistant diabetes mellitus, and atherosclerosis. WRN, the gene responsible for the disease, encodes a RECQL-type DNA helicase with a role in telomere metabolism. We derived a stable iPSC line from 53 years old patient's PBMC, with a normal karyotype, but exhibiting a short telomere length, as a major aspect of the cellular phenotype involved in the pathology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Flow Cytometry
  • Fluorescent Antibody Technique
  • Genetic Predisposition to Disease / genetics
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Karyotyping
  • Leukocytes, Mononuclear / cytology*
  • Leukocytes, Mononuclear / metabolism
  • Microsatellite Repeats / genetics
  • Telomere / genetics
  • Werner Syndrome / genetics*