Abstract
A cyclodipeptide synthase-containing gene cluster (pcm) was identified in Streptomyces chrestomyceticus NA4264 through genome mining. Heterologous expression of the cryptic pcm gene cluster in Streptomyces albus led to the production of a new highly modified cyclodipeptide named purincyclamide (1). Bioinformatic analysis and gene knockout experiments revealed the biosynthetic pathway. The production of 1 was achieved through a one-pot enzymatic reaction.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Molecular Structure
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Peptide Synthases / chemistry
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Peptide Synthases / genetics
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Peptide Synthases / metabolism*
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Purines / biosynthesis*
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Purines / chemistry
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Purines / isolation & purification
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Streptomyces / enzymology*
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Sulfonylurea Compounds / chemistry
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Sulfonylurea Compounds / isolation & purification
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Sulfonylurea Compounds / metabolism*
Substances
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Purines
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Sulfonylurea Compounds
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tolcyclamide
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Peptide Synthases
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purine
Supplementary concepts
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Streptomyces chrestomyceticus