Generation of seven iPSC lines from peripheral blood mononuclear cells suitable to investigate Autism Spectrum Disorder

Kiymet Bozaoglu; Yujing Gao; Edouard Stanley; Miriam Fanjul-Fernández; Natasha J Brown; Kate Pope; Cherie C Green; Katerina Vlahos; Koula Sourris; Melanie Bahlo; Martin Delatycki; Ingrid Scheffer; Paul J Lockhart

doi:10.1016/j.scr.2019.101516

Generation of seven iPSC lines from peripheral blood mononuclear cells suitable to investigate Autism Spectrum Disorder

Stem Cell Res. 2019 Aug:39:101516. doi: 10.1016/j.scr.2019.101516. Epub 2019 Aug 1.

Authors

Affiliations

¹ Bruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia; Department of Paediatrics, University of Melbourne, Parkville, Australia.
² Department of Paediatrics, University of Melbourne, Parkville, Australia; Murdoch Children's Research Institute, Parkville, Australia; Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria 3800, Australia.
³ Bruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia; Department of Paediatrics, University of Melbourne, Parkville, Australia; Victorian Clinical Genetics Services, Victoria, Australia.
⁴ Victorian Clinical Genetics Services, Victoria, Australia.
⁵ Bruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia.
⁶ Olga Tennison Autism Research Centre, School of Psychology and Public Health, La Trobe University, Bundoora, Victoria, Australia; Department of Medicine, Austin Health, The University of Melbourne, Heidelberg, Victoria, Australia.
⁷ Murdoch Children's Research Institute, Parkville, Australia.
⁸ The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Department of Medical Biology, University of Melbourne, Melbourne, Australia.
⁹ Department of Paediatrics, University of Melbourne, Parkville, Australia; Department of Medicine, University of Melbourne, Austin Health, Melbourne, VIC, Australia; Florey Institute, Melbourne, VIC, Australia; Department of Neurology, Royal Children's Hospital, Melbourne, Australia.
¹⁰ Bruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia; Department of Paediatrics, University of Melbourne, Parkville, Australia. Electronic address: [email protected].

PMID: 31415975
DOI: 10.1016/j.scr.2019.101516

Abstract

We have generated and characterized seven human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) from a single family, including unaffected and affected individuals clinically diagnosed with Autism Spectrum Disorder (ASD). The reprogramming of the PBMCs was performed using non-integrative Sendai virus containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. All iPSC lines exhibited a normal karyotype and pluripotency was validated by immunofluorescence, flow cytometry and their ability to differentiate into the three embryonic germ layers. These iPSC lines are a valuable resource to study the molecular mechanisms underlying ASD.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Autism Spectrum Disorder / metabolism*
Cellular Reprogramming / genetics
Cellular Reprogramming / physiology
Female
Flow Cytometry
Humans
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / metabolism
Kruppel-Like Factor 4
Kruppel-Like Transcription Factors / genetics
Kruppel-Like Transcription Factors / metabolism
Leukocytes, Mononuclear / cytology*
Leukocytes, Mononuclear / metabolism
Male
Octamer Transcription Factor-3 / genetics
Octamer Transcription Factor-3 / metabolism
SOXB1 Transcription Factors / genetics
SOXB1 Transcription Factors / metabolism
Sendai virus / genetics
Young Adult

Substances

KLF4 protein, human
Kruppel-Like Factor 4
Kruppel-Like Transcription Factors
Octamer Transcription Factor-3
POU5F1 protein, human
SOX2 protein, human
SOXB1 Transcription Factors