Abstract
Triggering antibody-mediated innate immune mechanisms to kill cancer cells is an attractive therapeutic avenue. In this context, recruitment of endogenous antibodies to the cancer cell surface could be a viable alternative to the use of monoclonal antibodies. We report on antibody-recruiting polymers containing multiple antibody-binding hapten motifs and cyclooctynes that can covalently conjugate to azides introduced onto the glycocalyx of cancer cells by metabolic labeling with azido sugars.
MeSH terms
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Acrylic Resins / chemical synthesis
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Acrylic Resins / chemistry*
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Animals
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Antibodies / immunology*
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Azides / chemistry
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Azides / metabolism*
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Cell Line, Tumor
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Click Chemistry
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Cycloaddition Reaction
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Cyclooctanes / chemical synthesis
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Cyclooctanes / chemistry
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Dinitrobenzenes / chemical synthesis
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Dinitrobenzenes / chemistry
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Dinitrobenzenes / immunology*
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Fluorescence
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Fluorescent Dyes / chemistry
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Glycocalyx / metabolism
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Hexosamines / chemistry
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Hexosamines / metabolism*
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Humans
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Mice
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Microscopy, Confocal / methods
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Microscopy, Fluorescence / methods
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Proof of Concept Study
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Spheroids, Cellular / metabolism
Substances
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Acrylic Resins
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Antibodies
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Azides
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Cyclooctanes
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Dinitrobenzenes
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Fluorescent Dyes
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Hexosamines
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N-azidoacetylmannosamine