Three new phenolic glycosides, intermedia A-C (1-3), one new acyclic alcohol glycoside, intermedia D (4), together with 3 known glycosides (5-7), were isolated from the dried roots of Paeonia intermedia C. A. Meyer. Their structures were established by means of extensive spectroscopic analysis (HRESIMS, NMR). Compound 1 have a rare benzo[1,5]dioxepine skeleton. The bioassay results showed that compound 3 exhibited inhibitory activity against proinflammatory cytokines nitric oxide (NO) secretion in LPS-activated RAW264.7 cells with an IC50 value of 85.76 ± 1.36 μM.
Keywords: Paeonia intermedia C. A. Meyer; acyclic alcohol glycoside; anti-inflammatory activity; phenolic glycoside.