Nearly Complete Genome Sequence of a Novel Phlebovirus-Like Virus Detected in a Human Plasma Sample by High-Throughput Sequencing

Florian Laubscher; Samuel Cordey; Mary-Anne Hartley; Gael Vieille; Noémie Boillat-Blanco; Josephine Samaka; Tarsis Mlaganile; Valerie d'Acremont; Laurent Kaiser

doi:10.1128/MRA.00764-19

Nearly Complete Genome Sequence of a Novel Phlebovirus-Like Virus Detected in a Human Plasma Sample by High-Throughput Sequencing

Microbiol Resour Announc. 2019 Aug 29;8(35):e00764-19. doi: 10.1128/MRA.00764-19.

Authors

Florian Laubscher^#^{1

2}, Samuel Cordey^#^{3

2}, Mary-Anne Hartley⁴, Gael Vieille^{1

2}, Noémie Boillat-Blanco^{5

6}, Josephine Samaka⁷, Tarsis Mlaganile⁷, Valerie d'Acremont^{4

5}, Laurent Kaiser^{1

2

8}

Affiliations

¹ Laboratory of Virology, University Hospitals of Geneva, Geneva, Switzerland.
² University of Geneva Medical School, Geneva, Switzerland.
³ Laboratory of Virology, University Hospitals of Geneva, Geneva, Switzerland [email protected].
⁴ Centre for Primary Care and Public Health (Unisanté), University of Lausanne, Lausanne, Switzerland.
⁵ Swiss Tropical and Public Health Institute, University of Basel, Basel, Switzerland.
⁶ Infectious Diseases Service, Lausanne University Hospital, Lausanne, Switzerland.
⁷ Ifakara Health Institute, Dar es Salaam, United Republic of Tanzania.
⁸ Geneva Centre for Emerging Viral Diseases, University Hospitals of Geneva, Geneva, Switzerland.

^# Contributed equally.

Abstract

Here, we report a novel phlebovirus-like virus sequence detected in a plasma sample from a febrile adult patient collected in the United Republic of Tanzania in 2014. A nearly complete RNA sequence was generated by high-throughput sequencing on a HiSeq 2500 instrument and further confirmed after repeating the analysis, starting from the initial sample.