Abstract
The activity of rifampin (RIF) and piperine was evaluated at the relative transcript levels of 12 efflux pumps (EPs), and an additional mechanism was proposed to be behind the synergic interactions of piperine plus RIF in Mycobacterium tuberculosis AutoDock v4.2.3 and Molegro v6 programs were used to evaluate PIP binding in M. tuberculosis RNA polymerase (RNAP). A hypothesis has been raised that piperine interferes in M. tuberculosis growth through RNAP inhibition, differently from what was previously endorsed for EP inhibition only.
Keywords:
RNA polymerase; docking; efflux pumps; piperine; rifampin; tuberculosis.
Copyright © 2019 American Society for Microbiology.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Alkaloids / administration & dosage
-
Alkaloids / metabolism
-
Alkaloids / pharmacology*
-
Antineoplastic Agents / administration & dosage
-
Antineoplastic Agents / metabolism
-
Antineoplastic Agents / pharmacology*
-
Benzodioxoles / administration & dosage
-
Benzodioxoles / metabolism
-
Benzodioxoles / pharmacology*
-
Binding Sites
-
DNA-Directed RNA Polymerases / metabolism*
-
Drug Synergism
-
Drug Therapy, Combination
-
Molecular Docking Simulation
-
Mycobacterium tuberculosis / drug effects*
-
Mycobacterium tuberculosis / enzymology
-
Mycobacterium tuberculosis / metabolism
-
Piperidines / administration & dosage
-
Piperidines / metabolism
-
Piperidines / pharmacology*
-
Polyunsaturated Alkamides / administration & dosage
-
Polyunsaturated Alkamides / metabolism
-
Polyunsaturated Alkamides / pharmacology*
-
Reverse Transcriptase Polymerase Chain Reaction
-
Rifampin / administration & dosage
-
Rifampin / metabolism
-
Rifampin / pharmacology*
Substances
-
Alkaloids
-
Antineoplastic Agents
-
Benzodioxoles
-
Piperidines
-
Polyunsaturated Alkamides
-
DNA-Directed RNA Polymerases
-
piperine
-
Rifampin