The transcribed pseudogene RPSAP52 enhances the oncofetal HMGA2-IGF2BP2-RAS axis through LIN28B-dependent and independent let-7 inhibition

Nat Commun. 2019 Sep 4;10(1):3979. doi: 10.1038/s41467-019-11910-6.

Abstract

One largely unknown question in cell biology is the discrimination between inconsequential and functional transcriptional events with relevant regulatory functions. Here, we find that the oncofetal HMGA2 gene is aberrantly reexpressed in many tumor types together with its antisense transcribed pseudogene RPSAP52. RPSAP52 is abundantly present in the cytoplasm, where it interacts with the RNA binding protein IGF2BP2/IMP2, facilitating its binding to mRNA targets, promoting their translation by mediating their recruitment on polysomes and enhancing proliferative and self-renewal pathways. Notably, downregulation of RPSAP52 impairs the balance between the oncogene LIN28B and the tumor suppressor let-7 family of miRNAs, inhibits cellular proliferation and migration in vitro and slows down tumor growth in vivo. In addition, high levels of RPSAP52 in patient samples associate with a worse prognosis in sarcomas. Overall, we reveal the roles of a transcribed pseudogene that may display properties of an oncofetal master regulator in human cancers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Breast Neoplasms / genetics
  • Breast Neoplasms / metabolism
  • Breast Neoplasms / therapy
  • Cell Line
  • Cell Line, Tumor
  • Female
  • Gene Expression Profiling / methods
  • Gene Expression Regulation, Neoplastic*
  • HMGA2 Protein / genetics
  • HMGA2 Protein / metabolism
  • Humans
  • Kaplan-Meier Estimate
  • MCF-7 Cells
  • Mice, Nude
  • MicroRNAs / genetics*
  • Proteins / genetics*
  • Proteins / metabolism
  • Pseudogenes / genetics*
  • RNA-Binding Proteins / genetics*
  • RNA-Binding Proteins / metabolism
  • RNAi Therapeutics / methods
  • Signal Transduction / genetics*
  • Transcription, Genetic
  • Tumor Burden / genetics
  • Xenograft Model Antitumor Assays / methods
  • ras Proteins / genetics
  • ras Proteins / metabolism

Substances

  • HMGA2 Protein
  • IGF2BP2 protein, human
  • LIN28B protein, human
  • MicroRNAs
  • Proteins
  • RNA-Binding Proteins
  • mirnlet7 microRNA, human
  • ras Proteins