Relationship between serum tumor markers and Anaplastic Lymphoma Kinase mutations in stage IV lung adenocarcinoma in Hubei province, Central China

J Clin Lab Anal. 2020 Jan;34(1):e23027. doi: 10.1002/jcla.23027. Epub 2019 Sep 6.

Abstract

Objective: The aim of this study was to explore the predictive value of carcinoembryonic antigen (CEA), squamous cell carcinoma antigen (SCCAg), and neuron-specific enolase (NSE) in the prediction of anaplastic lymphoma kinase (ALK) mutations in advance stage non-small cell lung cancer (NSCLC).

Subjects and methods: A total of 482 cases with untreated lung adenocarcinoma were retrospectively reviewed. Finally, 72 patients with stage IV were enrolled because of intact data of the detection of ALK rearrangement and serum tumor markers, as well they have not received any previous anticancer therapy. We used the one-way ANOVA analysis, correlation analysis, and multiple logistic regression analysis to evaluate the relationship between the level of serum tumor markers and ALK mutations.

Results: Fifteen cases with ALK mutations and 57 cases without mutations were identified. The result of the one-way ANOVA analysis showed only CEA was significantly associated with ALK mutations (95% CI:39.05-148.88; P = .001). The area under the ROC curve (AUC) of CEA was 0.705 (95%CI:0.567-0.843; P = .015). However, no significant association was observed between CEA and ALK mutations though the result of correlation analysis (P = .069) and multivariate logistic regression analysis (OR = 0.988, 95% CI: 0.972-1.003, P = .111).

Conclusions: In our study, we performed on the patients with stage IV lung adenocarcinoma in our region and found preoperative serum levels of SCCAg, CYRF21-1, and NSE not suitable for the detection of ALK mutation. Although we observed a significant association between CEA and ALK mutations; however, it was not strong enough to distinguish ALK status for the patients in our region.

Keywords: CYFRA21-1; anaplastic lymphoma kinase; carcinoembryonic antigen; neuron-specific enolase; squamous cell carcinoma antigen.

MeSH terms

  • Adenocarcinoma of Lung / blood*
  • Adenocarcinoma of Lung / enzymology
  • Adenocarcinoma of Lung / genetics*
  • Adult
  • Aged
  • Anaplastic Lymphoma Kinase / genetics*
  • Biomarkers, Tumor / blood*
  • China
  • Female
  • Humans
  • Lung Neoplasms / blood*
  • Lung Neoplasms / enzymology
  • Lung Neoplasms / genetics*
  • Male
  • Middle Aged
  • Multivariate Analysis
  • Mutation / genetics*
  • Neoplasm Staging
  • ROC Curve

Substances

  • Biomarkers, Tumor
  • ALK protein, human
  • Anaplastic Lymphoma Kinase