In vivo analysis of renal epithelial cells in zebrafish

Methods Cell Biol. 2019:154:163-181. doi: 10.1016/bs.mcb.2019.04.016. Epub 2019 Jun 14.

Abstract

The zebrafish kidney has been used effectively for studying kidney development, repair and disease. New gene editing capability makes it a more versatile in vivo vertebrate model system to investigate renal epithelial cells in their native environment. In this chapter we focus on dissecting gene function in basic cellular biology of renal epithelial cells, including lumen formation and cell polarity, in intact zebrafish embryos.

Keywords: Apical-basal polarity; CRISPR; Cilia; Epithelial cells; Kidney; Zebrafish.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • CRISPR-Associated Protein 9 / genetics
  • CRISPR-Associated Protein 9 / metabolism
  • CRISPR-Cas Systems
  • Cell Polarity
  • Cilia / metabolism
  • Cilia / ultrastructure
  • Embryo, Nonmammalian / anatomy & histology
  • Embryo, Nonmammalian / cytology*
  • Embryo, Nonmammalian / metabolism
  • Epithelial Cells / cytology*
  • Epithelial Cells / metabolism
  • Female
  • Gene Editing / methods*
  • Immunohistochemistry / methods*
  • Kidney / cytology*
  • Kidney / embryology
  • Kidney / metabolism
  • Male
  • Organogenesis / genetics
  • RNA, Guide, CRISPR-Cas Systems / genetics
  • RNA, Guide, CRISPR-Cas Systems / metabolism
  • Zebrafish
  • Zebrafish Proteins / deficiency
  • Zebrafish Proteins / genetics*

Substances

  • RNA, Guide, CRISPR-Cas Systems
  • Zebrafish Proteins
  • CRISPR-Associated Protein 9