Sequence-specific chemical modification of double-stranded DNA with alkylating oligodeoxyribonucleotide derivatives

V V Vlassov; S A Gaidamakov; V F Zarytova; D G Knorre; A S Levina; A A Nikonova; L M Podust; O S Fedorova

doi:10.1016/0378-1119(88)90158-8

Sequence-specific chemical modification of double-stranded DNA with alkylating oligodeoxyribonucleotide derivatives

Gene. 1988 Dec 10;72(1-2):313-22. doi: 10.1016/0378-1119(88)90158-8.

Authors

V V Vlassov¹, S A Gaidamakov, V F Zarytova, D G Knorre, A S Levina, A A Nikonova, L M Podust, O S Fedorova

Affiliation

¹ Institute of Bioorganic Chemistry, Siberian Division of the Academy of Sciences of U.S.S.R., Novosibirsk.

PMID: 3149602
DOI: 10.1016/0378-1119(88)90158-8

Abstract

Chemical modification of double-stranded (ds) DNA with alkylating oligodeoxynucleotide (oligo) derivatives, 5'-p(N-2-chloroethyl-N-methylamino) benzylamides of oligos, has been investigated. In contrast to relaxed plasmid DNAs, the superhelical molecules interact with the oligo derivatives and specific alkylation of the DNAs occurs at the regions complementary to the oligo reagents. Alkylating derivatives of oligocytidylates and pT(pCpT)6 react with corresponding homopyrimidine-homopurine tracts within ds DNA fragments due to triple helix formation.

MeSH terms

Alkylation
Animals
Base Sequence
Chemical Phenomena
Chemistry
DNA, Superhelical*
Gene Rearrangement
Genes, Immunoglobulin
Immunoglobulin Light Chains / genetics
Mice
Molecular Sequence Data
Mustard Compounds
Oligodeoxyribonucleotides*
Plasmids*

Substances

DNA, Superhelical
Immunoglobulin Light Chains
Mustard Compounds
Oligodeoxyribonucleotides