Generation of iPSC line (GIBHi001-A) from a patient with autism spectrum disorder

Shuai Li; Huifang Zhao; Rongqi Huang; Lang He; Chao Tian; Hualin Huang; Xiaobo Han; Feng Tang; Zuoxian Lin; Sihao Deng; Jianda Zhou; Zhiyuan Li

doi:10.1016/j.scr.2019.101571

Generation of iPSC line (GIBHi001-A) from a patient with autism spectrum disorder

Stem Cell Res. 2019 Oct:40:101571. doi: 10.1016/j.scr.2019.101571. Epub 2019 Sep 6.

Authors

Shuai Li¹, Huifang Zhao², Rongqi Huang¹, Lang He³, Chao Tian², Hualin Huang¹, Xiaobo Han¹, Feng Tang¹, Zuoxian Lin¹, Sihao Deng³, Jianda Zhou³, Zhiyuan Li⁴

Affiliations

¹ CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell. and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; University of Chinese Academy of Sciences, Beijing 100049, China; Guangzhou Regenerative Medicine and Health Guangdong Laboratory, 510005 Guangzhou, China.
² School of Life Sciences, University of Science and Technology of China, Hefei, China; Guangzhou Regenerative Medicine and Health Guangdong Laboratory, 510005 Guangzhou, China.
³ Department of Anatomy and Neurobiology, Xiangya School of Medicine, Central South University, Changsha, Hunan, China.
⁴ CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell. and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; School of Life Sciences, University of Science and Technology of China, Hefei, China; GZMU-GIBH Joint School of Life Sciences, Guangzhou Medical University, China; Department of Anatomy and Neurobiology, Xiangya School of Medicine, Central South University, Changsha, Hunan, China; University of Chinese Academy of Sciences, Beijing 100049, China; Guangzhou Regenerative Medicine and Health Guangdong Laboratory, 510005 Guangzhou, China. Electronic address: [email protected].

PMID: 31520889
DOI: 10.1016/j.scr.2019.101571

Abstract

Autism spectrum disorder (ASD) is a neurological disorder with complex etiologies. In this study, urine cells were collected from a 16-year-old male with ASD and reprogrammed with the human SKOM transcription factors. The patient has a heterozygous C > T mutation of FCGR1B gene that was confirmed by sequencing analysis. The pluripotency was verified by gene expression and capacity of differentiation towards the three germ layers. This kind of iPSC will be valuable for further understanding the pathogenesis of ASD and help to develop drugs for treating ASD.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Autism Spectrum Disorder / genetics
Cell Differentiation
Cell Line / cytology*
Cell Line / metabolism
Cells, Cultured
Heterozygote
Humans
Induced Pluripotent Stem Cells / cytology
Induced Pluripotent Stem Cells / metabolism*
Male
Mutation
Receptors, IgG / genetics
Receptors, IgG / metabolism

Substances

Receptors, IgG