Immunophenotyping of Circulating Endothelial Cells and Endothelial Microparticles

Nicholas Wanner; Kewal Asosingh

doi:10.1007/978-1-4939-9650-6_12

Immunophenotyping of Circulating Endothelial Cells and Endothelial Microparticles

Methods Mol Biol. 2019:2032:203-211. doi: 10.1007/978-1-4939-9650-6_12.

Authors

Nicholas Wanner¹, Kewal Asosingh²

Affiliations

¹ Department of Inflammation and Immunity, Cleveland Clinic, Lerner Research Institute, Cleveland, OH, USA.
² Department of Inflammation and Immunity, Cleveland Clinic, Lerner Research Institute, Cleveland, OH, USA. [email protected].

PMID: 31522421
DOI: 10.1007/978-1-4939-9650-6_12

Abstract

Flow-cytometric detection of circulating endothelial cells and endothelial microparticles is an essential tool in studies of vascular diseases. Here we describe the principles and detailed methods for human blood sample processing, storage, labeling, and gating of circulating endothelial elements.

Keywords: Endothelial; Flow cytometry; Immunophenotyping; Microparticle.

MeSH terms

Blood Platelets / immunology
Cell Count
Cell-Derived Microparticles / immunology
Endothelial Cells / immunology*
Flow Cytometry / methods*
Humans
Immunophenotyping / methods*
Vascular Diseases / diagnosis*
Vascular Diseases / immunology