Cellular Substrates for Cell-Based Tissue Engineering of Human Corneal Endothelial Cells

Int J Med Sci. 2019 Jul 21;16(8):1072-1077. doi: 10.7150/ijms.34440. eCollection 2019.

Abstract

Corneal endothelial tissue engineering aims to find solutions for blindness due to endothelial dysfunction. A suitable combination of endothelial cells, substrates and environmental cues should be deployed for engineering functional endothelial tissues. This manuscript reviews up-to-date topics of corneal endothelial tissue engineering with special emphasis on biomaterial substrates and their properties, efficacy, and mechanisms of supporting functional endothelial cells in vitro.

Keywords: collagen; endothelium; focal adhesion kinase; integrin; leukemia inhibitory factor; substrate; tissue engineering.

Publication types

  • Review

MeSH terms

  • Cell Culture Techniques
  • Cells, Cultured
  • Collagen / physiology
  • Endothelial Cells
  • Endothelium, Corneal* / cytology
  • Fibrillar Collagens / metabolism
  • Focal Adhesion Protein-Tyrosine Kinases / metabolism
  • Humans
  • Integrins / physiology
  • STAT3 Transcription Factor / metabolism
  • Signal Transduction
  • Tissue Engineering / methods*

Substances

  • Fibrillar Collagens
  • Integrins
  • STAT3 Transcription Factor
  • STAT3 protein, human
  • atelocollagen
  • Collagen
  • Focal Adhesion Protein-Tyrosine Kinases