miR-671-5p Blocks The Progression Of Human Esophageal Squamous Cell Carcinoma By Suppressing FGFR2

Xiaoyan Li; Changjun Nie; Baoqing Tian; Xuan Tan; Wei Han; Jiakang Wang; Yuan Jin; Yadan Li; Xinyuan Guan; An Hong; Xiaojia Chen

doi:10.7150/ijbs.32429

miR-671-5p Blocks The Progression Of Human Esophageal Squamous Cell Carcinoma By Suppressing FGFR2

Int J Biol Sci. 2019 Jul 21;15(9):1892-1904. doi: 10.7150/ijbs.32429. eCollection 2019.

Authors

Xiaoyan Li^{1

2

3}, Changjun Nie^{1

2

3}, Baoqing Tian^{1

2

3}, Xuan Tan^{1

2

3}, Wei Han^{1

2

3}, Jiakang Wang⁴, Yuan Jin^{1

2

3}, Yadan Li^{1

2

3}, Xinyuan Guan⁵, An Hong^{1

2

3}, Xiaojia Chen^{1

2

3}

Affiliations

¹ Institute of Biomedicine & Department of cell Biology, Jinan University, Guangzhou, Guangdong, 510632, P. R. China.
² National Engineering Research Center of Genetic Medicine, Guangzhou, Guangdong, 510632, P. R. China.
³ Guangdong Provincial Key Laboratory of Bioengineering Medicine, Guangzhou, Guangdong, 510632, P. R. China.
⁴ Cancer Center of Guangzhou Medical University, Guangzhou, Guangdong, 510090, P. R. China.
⁵ Department of Clinical Oncology, University of Hong Kong, Hong Kong, China.

Abstract

Esophageal cancer is the eighth most common malignant tumor worldwide, of which esophageal squamous cell carcinoma (ESCC) is the dominant histological subtype. A drug shortage for ESCC therapy triggered us to explore the roles of fibroblast growth factor receptor 2 (FGFR2) and its upstream regulator miR-671-5p in ESCC progression. We compared the levels of FGFR2 and miR-671-5p between human ESCC tissues and their matched normal esophageal tissues and found an association between higher levels of FGFR2 and lower levels of miR-671-5p in ESCC tissues. High levels of FGFR2 resulted in the activation of the ERK and AKT pathways and a promotion of ESCC progression. High levels of miR-671-5p specifically reduced the expression of FGFR2 and suppressed ESCC progression in both in vitro and in vivo models. Therefore, suppressing FGFR2 and enhancing miR-671-5p expression may be the right approaches for ESCC therapy.

Keywords: ESCC; FGFR2; miR-671-5p; tumor progression.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3' Untranslated Regions / genetics
Animals
Cell Line, Tumor
Cell Movement / genetics
Cell Movement / physiology
Cell Proliferation / genetics
Cell Proliferation / physiology
Disease Progression
Esophageal Neoplasms / genetics
Esophageal Neoplasms / metabolism*
Esophageal Neoplasms / pathology
Esophageal Squamous Cell Carcinoma / genetics
Esophageal Squamous Cell Carcinoma / metabolism*
Esophageal Squamous Cell Carcinoma / pathology
Gene Expression Regulation, Neoplastic / genetics
Gene Expression Regulation, Neoplastic / physiology
Humans
Immunoblotting
In Vitro Techniques
Mice
Mice, Inbred BALB C
MicroRNAs / genetics
MicroRNAs / metabolism*
Receptor, Fibroblast Growth Factor, Type 2 / genetics
Receptor, Fibroblast Growth Factor, Type 2 / metabolism*
Signal Transduction / genetics
Signal Transduction / physiology

Substances

3' Untranslated Regions
MIRN671 microRNA, human
MicroRNAs
FGFR2 protein, human
Receptor, Fibroblast Growth Factor, Type 2