β2 Adrenergic Receptor Complexes with the L-Type Ca2+ Channel CaV1.2 and AMPA-Type Glutamate Receptors: Paradigms for Pharmacological Targeting of Protein Interactions

Annu Rev Pharmacol Toxicol. 2020 Jan 6:60:155-174. doi: 10.1146/annurev-pharmtox-010919-023404. Epub 2019 Sep 27.

Abstract

Formation of signaling complexes is crucial for the orchestration of fast, efficient, and specific signal transduction. Pharmacological disruption of defined signaling complexes has the potential for specific intervention in selected regulatory pathways without affecting organism-wide disruption of parallel pathways. Signaling by epinephrine and norepinephrine through α and β adrenergic receptors acts on many signaling pathways in many cell types. Here, we initially provide an overview of the signaling complexes formed between the paradigmatic β2 adrenergic receptor and two of its most important targets, the L-type Ca2+ channel CaV1.2 and the AMPA-type glutamate receptor. Importantly, both complexes contain the trimeric Gs protein, adenylyl cyclase, and the cAMP-dependent protein kinase, PKA. We then discuss the functional implications of the formation of these complexes, how those complexes can be specifically disrupted, and how such disruption could be utilized in the pharmacological treatment of disease.

Keywords: AKAP; Gs; PSD-95; adenylyl cyclase; cAMP; norepinephrine.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Animals
  • Calcium Channels, L-Type / drug effects
  • Calcium Channels, L-Type / metabolism*
  • Cyclic AMP-Dependent Protein Kinases / metabolism
  • Epinephrine / metabolism
  • Humans
  • Norepinephrine / metabolism
  • Receptors, AMPA / drug effects
  • Receptors, AMPA / metabolism*
  • Receptors, Adrenergic, beta-2 / drug effects
  • Receptors, Adrenergic, beta-2 / metabolism*
  • Signal Transduction / drug effects

Substances

  • CACNA1C protein, human
  • Calcium Channels, L-Type
  • Receptors, AMPA
  • Receptors, Adrenergic, beta-2
  • Cyclic AMP-Dependent Protein Kinases
  • Norepinephrine
  • Epinephrine