MRSA dynamic circulation between the community and the hospital setting: New insights from a cohort study

J Infect. 2020 Jan;80(1):24-37. doi: 10.1016/j.jinf.2019.10.001. Epub 2019 Oct 11.

Abstract

Dissemination of methicillin-resistant-Staphylococcus aureus/(MRSA) is a worldwide concern both in hospitals [healthcare-associated-(HA)-MRSA] and communities [community-associated-(CA)-MRSA]. Knowledge on when and where MRSA colonization is acquired and what clones are involved is necessary, to focus efforts for prevention of hospital-acquired MRSA-infections.

Methods: A prospective/longitudinal cohort study was performed in eight Argentina hospitals (Cordoba/ October-December/2014). Surveillance cultures for MRSA (nose-throat-inguinal) were obtained on admission and at discharge. MRSA strains were genetically typed as CA-MRSAG and HA-MRSAG genotypes.

Results: Overall, 1419 patients were screened and 534 stayed at hospital for ≥3 days. S. aureus admission prevalence was 30.9% and 4.2% for MRSA. Overall MRSA acquisition rate was 2.3/1000 patient-days-at-risk with a MRSA acquisition prevalence of 1.96% (95%CI: 1.0%-3.4%); 3.2% of patients were discharged back to community with MRSA. CA-MRSAG accounted for 84.6% of imported, 100.0% of hospital-acquired and 94% of discharged MRSA strains. Most imported and acquired MRSA strains belonged to two major epidemic CA-MRSA clones spread in Argentina: PFGEtypeI-ST5-IVa-t311-PVL+ and PFGEtypeN/ST30-IVc-t019-PVL+.

Conclusions: CA-MRSA clones, particularly ST5-IV-PVL+ and ST30-IV-PVL+, with main reservoir in the community, not only enter but also are truly acquired within hospital, causing healthcare-associated-hospital-onset infections, having a transmission capacity greater or similar than HA-MRSAG. This information is essential to develop appropriate MRSA infection prevention-control programs, considering hospital and community.

Keywords: Argentina; CA-MRSA; Hospital-acquired infections; MRSA; MRSA carriage; ST30-IV; ST5-IV.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cohort Studies
  • Community-Acquired Infections* / epidemiology
  • Cross Infection* / epidemiology
  • Exotoxins
  • Hospitals
  • Humans
  • Leukocidins
  • Longitudinal Studies
  • Methicillin-Resistant Staphylococcus aureus* / genetics
  • Prospective Studies
  • Staphylococcal Infections* / epidemiology
  • Staphylococcus aureus

Substances

  • Exotoxins
  • Leukocidins