Emergence of an NDM-5-Producing Hypervirulent Klebsiella pneumoniae Sequence Type 35 Strain with Chromosomal Integration of an Integrative and Conjugative Element, ICE Kp1

Antimicrob Agents Chemother. 2019 Dec 20;64(1):e01675-19. doi: 10.1128/AAC.01675-19. Print 2019 Dec 20.

Abstract

Here, we report an NDM-5-producing sequence type 35 (ST35) hypervirulent Klebsiella pneumoniae strain, isolated from the blood of a male patient. It showed a remarkable resistance to serum killing and neutrophil phagocytosis and high virulence in a mouse peritonitis infection model. Instead of carrying a pLVPK-like virulence plasmid, chromosomal integration of ICEKp1 (∼76 kb) was identified in a specific asparagine-tRNA gene, harboring the iron acquisition system salmochelin genes (iroBCDN), a yersiniabactin gene, and a variant of the rmpA gene.

Keywords: ICEKp1; NDM-5; ST35; carbapenem-resistant hypervirulent K. pneumoniae.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Female
  • Gene Transfer Techniques
  • Humans
  • Klebsiella Infections / genetics
  • Klebsiella pneumoniae / genetics*
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Peritonitis / genetics
  • Peritonitis / microbiology
  • Plasmids / genetics
  • Virulence

Substances

  • Bacterial Proteins
  • RmpA protein, Klebsiella pneumoniae