Exploiting MATE efflux proteins to improve flavonoid accumulation in Camellia sinensis in silico

Int J Biol Macromol. 2020 Jan 15:143:732-743. doi: 10.1016/j.ijbiomac.2019.10.028. Epub 2019 Oct 14.

Abstract

Flavonoids in tea plant are the important bioactive compounds for both human health and taste quality. Multidrug and Toxic compound Extrusion (MATE) proteins could improve flavonoid accumulations by transporting and sequestering the flavonoid in vacuoles. We identified 41 putative MATE genes in tea plants. The similar intron-exon structures of tea MATEs clustered within the same gene clade. The correlation analysis of tea flavonoid and transcriptome data showed that TEA006173 might be involve in the tea flavonoid accumulation. The RT-PCR results confirmed that TEA006173 showed high expression in the young leaf tissues. Tertiary structure prediction has shown that TEA006173 contained the 12 helices with three active pockets, comprising 13 critical residues. The present study provided the structural variations and expression patterns of tea MATEs and it would be helpful for taste and nutrient quality improvement in tea plant.

Keywords: Camellia sinensis; Flavonoids; MATE efflux proteins.

MeSH terms

  • Amino Acid Motifs
  • Amino Acid Sequence
  • Camellia sinensis / genetics
  • Camellia sinensis / metabolism*
  • Computer Simulation*
  • Conserved Sequence
  • Exons / genetics
  • Flavonoids / metabolism*
  • Gene Expression Regulation, Plant
  • Genome, Plant
  • Introns / genetics
  • Membrane Transport Proteins / chemistry
  • Membrane Transport Proteins / metabolism*
  • Phylogeny
  • Plant Leaves / genetics
  • Plant Leaves / growth & development
  • Plant Proteins / chemistry
  • Plant Proteins / genetics
  • Plant Proteins / metabolism*
  • Protein Structure, Tertiary
  • Transcriptome / genetics

Substances

  • Flavonoids
  • Membrane Transport Proteins
  • Plant Proteins