Purification and identification of antioxidant peptides from round scad (Decapterus maruadsi) hydrolysates by consecutive chromatography and electrospray ionization-mass spectrometry

Xiao Hu; Xianqing Yang; Tingting Wang; Laihao Li; Yanyan Wu; Ya Zhou; Lijun You

doi:10.1016/j.fct.2019.110882

Purification and identification of antioxidant peptides from round scad (Decapterus maruadsi) hydrolysates by consecutive chromatography and electrospray ionization-mass spectrometry

Food Chem Toxicol. 2020 Jan:135:110882. doi: 10.1016/j.fct.2019.110882. Epub 2019 Oct 14.

Authors

Xiao Hu¹, Xianqing Yang², Tingting Wang³, Laihao Li⁴, Yanyan Wu¹, Ya Zhou³, Lijun You⁵

Affiliations

¹ Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, China.
² Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, 222005, China. Electronic address: [email protected].
³ Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, China; College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China.
⁴ Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, 222005, China.
⁵ School of Food Science and Engineering, South China University of Technology, Guangzhou, 510640, China. Electronic address: [email protected].

PMID: 31622727
DOI: 10.1016/j.fct.2019.110882

Abstract

Round scad (Decapterus maruadsi) was hydrolyzed with a double-enzyme (a mixture of neutrase and trypsin) to obtain antioxidant peptides. The round scad hydrolysates obtained by 5-h hydrolysis (RSH) displayed the strongest antioxidant activities, which could scavenge the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, the hydroxyl radical, and exhibit reducing power. RSH was further separated into four fractions by using an ultrafiltration membrane system, and low-molecular-weight fraction RSH-IV (<5 kDa) showed the highest antioxidant activities. Fraction RSH-IV was then purified with gel filtration chromatography followed by reverse high-performance liquid chromatography (RP-HPLC). The sequence of the purified antioxidant peptide was identified as Lys-Gly-Phe-Arg (506 Da) by liquid chromatography/electrospray ionization tandem mass spectrometry (LC-MS/MS). Additionally, the purified peptide could scavenge DPPH radical at IC₅₀ value of 0.13 mg/mL, and it showed a 49.08-fold higher DPPH radical scavenging activity compared with that of the crude RSH. The results suggest that antioxidant peptides obtained from round scad (Decapterus maruadsi) could be a good source of natural antioxidant.

Keywords: Antioxidant peptides; Hydrolysis; Identification; Purification; Round scad (Decapterus maruadsi).

MeSH terms

Animals
Antioxidants / metabolism*
Chromatography, Liquid / methods*
Fish Proteins / metabolism*
Fishes / metabolism*
Hydrolysis
Peptides / metabolism*
Spectrometry, Mass, Electrospray Ionization / methods*
Tandem Mass Spectrometry / methods*

Substances

Antioxidants
Fish Proteins
Peptides