Directed Evolution of CRISPR-Cas9 Base Editors

Jackson Winter; Pablo Perez-Pinera

doi:10.1016/j.tibtech.2019.09.005

Directed Evolution of CRISPR-Cas9 Base Editors

Trends Biotechnol. 2019 Nov;37(11):1151-1153. doi: 10.1016/j.tibtech.2019.09.005. Epub 2019 Oct 14.

Authors

Jackson Winter¹, Pablo Perez-Pinera²

Affiliations

¹ Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
² Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Carle Illinois College of Medicine, Champaign, IL 61820, USA; Cancer Center at Illinois, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. Electronic address: [email protected].

PMID: 31623959
DOI: 10.1016/j.tibtech.2019.09.005

Abstract

A recent publication by Thuronyi et al. described a directed evolution system called phage-assisted continuous evolution (PACE) that was used to generate improved variants of CRISPR-Cas9 base editors. These evolved base editors overcome some of the inherent limitations of the technology such as sequence context preferences, restricted editing windows, and large construct sizes.

MeSH terms

CRISPR-Associated Protein 9 / genetics*
CRISPR-Cas Systems / genetics*
Clustered Regularly Interspaced Short Palindromic Repeats / genetics*
Evolution, Molecular
Gene Editing / methods

Substances

CRISPR-Associated Protein 9