Glucagon-like peptide-1 receptor agonist dulaglutide prevents ox-LDL-induced adhesion of monocytes to human endothelial cells: An implication in the treatment of atherosclerosis

Mol Immunol. 2019 Dec:116:73-79. doi: 10.1016/j.molimm.2019.09.021. Epub 2019 Oct 17.

Abstract

Atherosclerosis is a common comorbidity of type II diabetes and a leading cause of death worldwide. The presence of oxidized low-density lipoprotein (ox-LDL) drives atherogenesis by inducing oxidative stress, mitochondrial dysfunction, expression of proinflammatory cytokines and chemokines including interleukin (IL)-1β, IL-6, and monocyte chemoattractant protein 1 (MCP-1), adhesion molecules including vascular cellular adhesion molecule 1 (VCAM-1) and E-selectin, and downregulating expression of the Krüppel-like factor 2 (KLF2) transcription factor. Importantly, ox-LDL induced the attachment of THP-1 monocytes to endothelial cells. In the present study, we demonstrate for the first time that the specific glucagon-like peptide 1 receptor (GLP-1R) agonist dulaglutide may prevent these atherosclerotic effects of ox-LDL by preventing suppression of KLF2 by p53 protein in human aortic endothelial cells. KLF2 has been shown to play a major role in protecting vascular endothelial cells from damage induced by ox-LDL and oscillatory shear, and therefore, therapies capable of mediating KLF2 signaling may be an attractive treatment option for preventing the development and progression of atherosclerosis.

Keywords: Atherosclerosis; Krüppel-like factor 2; Oxidized low-density lipoprotein; Vascular cellular adhesion molecule 1 (VCAM-1).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Atherosclerosis / drug therapy*
  • Atherosclerosis / metabolism
  • Cell Adhesion
  • Cells, Cultured
  • Cytokines / metabolism
  • Diabetes Mellitus, Type 2 / metabolism
  • Endothelial Cells
  • Gene Expression Regulation / drug effects
  • Glucagon-Like Peptide-1 Receptor / metabolism*
  • Glucagon-Like Peptides / analogs & derivatives*
  • Glucagon-Like Peptides / pharmacology
  • Human Umbilical Vein Endothelial Cells / drug effects
  • Human Umbilical Vein Endothelial Cells / metabolism
  • Humans
  • Immunoglobulin Fc Fragments / pharmacology*
  • Kruppel-Like Transcription Factors / metabolism
  • Lipoproteins, LDL / metabolism*
  • Monocytes / drug effects*
  • Monocytes / metabolism
  • NF-kappa B / metabolism
  • Recombinant Fusion Proteins / pharmacology*
  • Signal Transduction / drug effects
  • Vascular Cell Adhesion Molecule-1 / metabolism

Substances

  • Cytokines
  • GLP1R protein, human
  • Glucagon-Like Peptide-1 Receptor
  • Immunoglobulin Fc Fragments
  • Kruppel-Like Transcription Factors
  • Lipoproteins, LDL
  • NF-kappa B
  • Recombinant Fusion Proteins
  • Vascular Cell Adhesion Molecule-1
  • oxidized low density lipoprotein
  • Glucagon-Like Peptides
  • dulaglutide