Abstract
In this study the ultrastructural localization of antigens recognized by novel antibodies that allow the recognition of lymphoid antigens in conventionally fixed and wax-embedded sections was investigated. MT1, MB1, and UCHL1 recognize antibodies restricted to the cell membrane, whereas the antigen recognized by MB2 is present only in the cytoplasm. These distributions are different from that of immunoreactivity with TAL 1B5 (anti-HLA DR), which is present both on the cell membrane and in the cytoplasm. The significance of these findings is discussed.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Monoclonal / analysis
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Antibodies, Monoclonal / immunology*
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Cell Membrane / metabolism
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Cell Membrane / ultrastructure
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Cytoplasm / metabolism
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Cytoplasm / ultrastructure
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HLA Antigens / analysis*
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HLA Antigens / immunology
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Histocompatibility Antigens Class II / analysis
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Histocompatibility Antigens Class II / immunology
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Humans
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Immunohistochemistry / methods
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Palatine Tonsil / cytology
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Palatine Tonsil / metabolism
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Palatine Tonsil / ultrastructure
Substances
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Antibodies, Monoclonal
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HLA Antigens
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Histocompatibility Antigens Class II