Wheat Yellow Mosaic Virus NIb Interacting with Host Light Induced Protein (LIP) Facilitates Its Infection through Perturbing the Abscisic Acid Pathway in Wheat

Tianye Zhang; Peng Liu; Kaili Zhong; Fan Zhang; Miaoze Xu; Long He; Peng Jin; Jianping Chen; Jian Yang

doi:10.3390/biology8040080

Wheat Yellow Mosaic Virus NIb Interacting with Host Light Induced Protein (LIP) Facilitates Its Infection through Perturbing the Abscisic Acid Pathway in Wheat

Biology (Basel). 2019 Oct 23;8(4):80. doi: 10.3390/biology8040080.

Authors

Tianye Zhang^{1

2}, Peng Liu³, Kaili Zhong⁴, Fan Zhang⁵, Miaoze Xu⁶, Long He⁷, Peng Jin⁸, Jianping Chen^{9

10}, Jian Yang¹¹

Affiliations

¹ School of Forestry and Biotechnology, Zhejiang Agriculture and Forestry University, Hangzhou 310021, China. [email protected].
² State Key Laboratory for Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo 315211, China. [email protected].
³ State Key Laboratory for Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo 315211, China. [email protected].
⁴ State Key Laboratory for Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo 315211, China. [email protected].
⁵ State Key Laboratory for Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo 315211, China. [email protected].
⁶ State Key Laboratory for Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo 315211, China. [email protected].
⁷ State Key Laboratory for Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo 315211, China. [email protected].
⁸ State Key Laboratory for Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo 315211, China. [email protected].
⁹ School of Forestry and Biotechnology, Zhejiang Agriculture and Forestry University, Hangzhou 310021, China. [email protected].
¹⁰ State Key Laboratory for Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo 315211, China. [email protected].
¹¹ State Key Laboratory for Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo 315211, China. [email protected].

Abstract

Positive-sense RNA viruses have a small genome with very limited coding capacity and are highly reliant on host factors to fulfill their infection. However, few host factors have been identified to participate in wheat yellow mosaic virus (WYMV) infection. Here, we demonstrate that wheat (Triticum aestivum) light-induced protein (TaLIP) interacts with the WYMV nuclear inclusion b protein (NIb). A bimolecular fluorescence complementation (BIFC) assay displayed that the subcellular distribution patterns of TaLIP were altered by NIb in Nicotiana benthamiana. Transcription of TaLIP was significantly decreased by WYMV infection and TaLIP-silencing wheat plants displayed more susceptibility to WYMV in comparison with the control plants, suggesting that knockdown of TaLIP impaired host resistance. Moreover, the transcription level of TaLIP was induced by exogenous abscisic acid (ABA) stimuli in wheat, while knockdown of TaLIP significantly repressed the expression of ABA-related genes such as wheat abscisic acid insensitive 5 (TaABI5), abscisic acid insensitive 8 (TaABI8), pyrabatin resistance 1-Llike (TaPYL1), and pyrabatin resistance 3-Llike (TaPYL3). Collectively, our results suggest that the interaction of NIb with TaLIP facilitated the virus infection possibly by disturbing the ABA signaling pathway in wheat.

Keywords: ABA signaling pathway; BIFC; wheat yellow mosaic virus.

Abstract

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