In vitro human cord blood platelet lysate characterisation with potential application in wound healing

Int Wound J. 2020 Feb;17(1):65-72. doi: 10.1111/iwj.13233. Epub 2019 Oct 30.

Abstract

Platelets contain abundant growth factors and cytokines that have a positive influence on the migration and proliferation of different cell types by modulating its physiopathological processes. As it is known that human umbilical cord blood platelet lysate (UCB-PL) contains a supraphysiological concentration of growth factors, in the present study, we investigated its effectiveness in wound-healing processes. Human UCB-PL was obtained by the freeze/thaw of platelet concentrate (1.1 × 109 platelets/L), and its effect was evaluated on human or mouse endothelial cells, monocytes, fibroblasts, and keratinocytes in different concentrations. Human UCB-PL was observed to have high levels of pro-angiogenic growth factor than peripheral blood platelet-rich plasma. Among the cell lines, different concentrations of human UCB-PL were necessary to influence their viability and proliferation. For L929 cells, 5% of total volume was necessary, while for human umbilical vein endothelial cell, it was 10%. Cell migration on monocytes was increased with respect to the positive control, and scratch closure on keratinocytes was increased with respect to serum-free medium with only 10% of human UCB-PL. We concluded that the human UCB-PL may be useful to produce a large amount of standard platelet concentrates sufficient for several clinical-scale expansions avoiding inter-individual variability, which can also be used as a functional tool for clinical regenerative application for wound healing.

Keywords: human umbilical cord blood; in vitro characterisation; platelet lysate; wound healing.

MeSH terms

  • Blood Platelets / chemistry*
  • Cell Proliferation / drug effects*
  • Cell Proliferation / physiology
  • Cells, Cultured / drug effects
  • Cytokines / therapeutic use*
  • Endothelial Growth Factors / therapeutic use*
  • Human Umbilical Vein Endothelial Cells / chemistry*
  • Humans
  • Platelet-Rich Plasma / chemistry*
  • Wound Healing / drug effects*
  • Wound Healing / physiology
  • Wounds and Injuries / drug therapy*

Substances

  • Cytokines
  • Endothelial Growth Factors