CART manufacturing process and reasons for academy-pharma collaboration

Immunol Lett. 2020 Jan:217:39-48. doi: 10.1016/j.imlet.2019.10.014. Epub 2019 Oct 25.

Abstract

The success of genetically engineered T-cells modified with a chimeric antigen receptor as an adoptive cell immunotherapy and the subsequent last regulatory approvals of products based on this therapy are leading to a crescent number of both academic and pharmaceutical industry clinical trials testing new approaches of this "living drugs". The aim of this review is to outline the latest developments and regulatory considerations in this field, with a particular emphasis to differences and similarities between academic and industry approaches and the role they should play to coexist and move forward together. To do that, the main considerations for the manufacturing process are firstly discussed, from the chimeric antigen receptor design to final production steps, passing through ex vivo T-cell handling, gene delivery methods, patient´s final product infusion observations or possible associated side effects of this treatment.

Keywords: Adoptive cell immunotherapy; CART; Chimeric antigen receptor; Good manufacturing practice.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Academies and Institutes
  • Biopharmaceutics / methods
  • Costs and Cost Analysis
  • Gene Editing
  • Genetic Engineering / methods
  • Genetic Therapy / methods
  • Genetic Vectors
  • Humans
  • Immunotherapy, Adoptive / adverse effects
  • Immunotherapy, Adoptive / legislation & jurisprudence
  • Immunotherapy, Adoptive / methods*
  • Immunotherapy, Adoptive / trends
  • Industry / economics
  • Industry / methods
  • Manufacturing Industry / economics
  • Neoplasms / drug therapy*
  • Neoplasms / immunology*
  • Receptors, Antigen, T-Cell / genetics
  • Receptors, Antigen, T-Cell / immunology*
  • T-Lymphocytes / cytology
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / metabolism

Substances

  • Receptors, Antigen, T-Cell