Molecular characterization of multidrug-resistance in Gram-negative bacteria from the Peshawar teaching hospital, Pakistan

A Masseron; L Poirel; B Jamil Ali; M A Syed; P Nordmann

doi:10.1016/j.nmni.2019.100605

Molecular characterization of multidrug-resistance in Gram-negative bacteria from the Peshawar teaching hospital, Pakistan

New Microbes New Infect. 2019 Oct 11:32:100605. doi: 10.1016/j.nmni.2019.100605. eCollection 2019 Nov.

Authors

A Masseron¹, L Poirel^{1

2

3}, B Jamil Ali⁴, M A Syed⁵, P Nordmann^{1

2

3

6}

Affiliations

¹ Medical and Molecular Microbiology, Section of Medicine, Faculty of Science and Medicine, Switzerland.
² INSERM European Unit (IAME, France), University of Fribourg, Fribourg, Switzerland.
³ Swiss National Reference Centre for Emerging Antibiotic Resistance, University of Fribourg, Switzerland.
⁴ Section of Infectious Diseases, Department of Medicine, The Aga Khan University, Karashi, Pakistan.
⁵ Infectious Diseases Research Group, Department of Microbiology, University of Haripur, Khyber Pakhtunkhwa, Pakistan.
⁶ Institute for Microbiology, University of Lausanne and University Hospital Centre, Lausanne, Switzerland.

Abstract

Extended-spectrum β-lactamases, carbapenemases, 16S rRNA methylases conferring pan-drug aminoglycoside resistance and colistin resistance were investigated among Gram-negative bacteria recovered from clinical samples (infections) from 200 individuals hospitalized at the Khyber Teaching Hospital of Peshawar, north Pakistan, from December 2017 to March 2018. Out of 65 isolates recovered, 19% were carbapenem resistant and 16% carried a bla _NDM-1 gene, confirming the widespread distribution of NDM producers in this country. The association of the NDM carbapenem-resistance determinant, together with the extended-spectrum β-lactamase CTX-M-15 and 16S rRNA methylases, was frequent, explaining the multidrug-resistance pattern observed. All isolates remained susceptible to colistin.

Keywords: 16S rRNA methylases; Carbapenemases; Extended spectrum β-lactamase; Pakistan; ST 215; ST 231.