Abstract
The linear synthesis of the N-terminal domain of mISG15 has been developed which enables the synthesis of full-length mISG15 and the activity-based probe Rho-mISG15-PA via native chemical ligation. Pilot experiments showed that the synthetic proteins were properly folded and recognized by endogenous enzymes. Our synthesis strategy allows the generation of other mISG15-based probes and reagents that can accelerate the research in this field.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Cytokines / chemical synthesis*
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Cytokines / metabolism*
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Fluorescent Dyes / chemistry
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Interleukins / metabolism
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Ligands
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Molecular Weight
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Protein Binding
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Protein Domains
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Rhodamines / chemistry
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Solid-Phase Synthesis Techniques / methods*
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Structure-Activity Relationship
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Ubiquitins / chemical synthesis
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Ubiquitins / metabolism
Substances
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Cytokines
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Fluorescent Dyes
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G1p2 protein, mouse
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Interleukins
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Ligands
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Rhodamines
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Ubiquitins