Abstract
We designed liposomes dually functionalized with ApoE-derived peptide (mApoE) and chlorotoxin (ClTx) to improve their blood-brain barrier (BBB) crossing. Our results demonstrated the synergistic activity of ClTx-mApoE in boosting doxorubicin-loaded liposomes across the BBB, keeping the anti-tumour activity of the drug loaded: mApoE acts promoting cellular uptake, while ClTx promotes exocytosis of liposomes.
Keywords:
Blood–brain barrier; Brain; Chlorotoxin; Doxorubicin; Drug delivery; Glioblastoma; Liposomes; Nanoparticles.
MeSH terms
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Animals
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Antibiotics, Antineoplastic / administration & dosage
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Antibiotics, Antineoplastic / pharmacokinetics*
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Antibiotics, Antineoplastic / pharmacology
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Apolipoproteins E / chemistry
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Apolipoproteins E / metabolism*
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Blood-Brain Barrier / metabolism*
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Brain Neoplasms / drug therapy
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Brain Neoplasms / metabolism
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Cell Line, Tumor
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Doxorubicin / administration & dosage
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Doxorubicin / analogs & derivatives*
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Doxorubicin / pharmacokinetics
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Doxorubicin / pharmacology
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Humans
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Liposomes / chemistry
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Liposomes / metabolism*
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Models, Molecular
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Peptides / chemistry
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Peptides / metabolism
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Polyethylene Glycols / administration & dosage
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Polyethylene Glycols / pharmacokinetics
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Polyethylene Glycols / pharmacology
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Scorpion Venoms / chemistry
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Scorpion Venoms / metabolism*
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Scorpions
Substances
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Antibiotics, Antineoplastic
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Apolipoproteins E
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Liposomes
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Peptides
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Scorpion Venoms
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liposomal doxorubicin
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Chlorotoxin
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Polyethylene Glycols
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Doxorubicin