Validation of the AGCU Expressmarker 16 + 22Y Kit: a new multiplex for forensic application

Int J Legal Med. 2020 Jan;134(1):177-183. doi: 10.1007/s00414-019-02200-3. Epub 2019 Nov 12.

Abstract

Genotyping by targeting short tandem repeats (STRs) has been widely used in forensic applications. However, most commercial kits detect autosomal STRs or Y-STRs alone, which waste both time and opportunity. The AGCU Expressmarker 16 + 22Y Kit includes 16 autosomal and 22 Y-chromosomal STR loci and is designed for the forensic science field and obtaining quicker results. Here, we conducted the validation study according to Scientific Working Group on DNA Analysis Methods (SWGDAM) guidelines. Validation of PCR-based studies, species specificity, sensitivity, DNA mixture studies, inhibitors, precision, and sizing accuracy were performed. Furthermore, this system was also tested in 346 random male samples from Han, Hui, Tibetan, and Zhuang populations in China, showing its high power for forensic discrimination in the Chinese population. In addition, this system was able to deal with AMELY deletion cases, which can correctly identify sex in forensic criminal investigations. Our results suggested that the AGCU Expressmarker 16 + 22Y Kit is a useful tool for rapid criminal investigation.

Keywords: AGCU Expressmarker 16 + 22Y Kit; Forensic science; Short tandem repeat (STR); Validation studies.

Publication types

  • Validation Study

MeSH terms

  • Animals
  • Blood
  • China / ethnology
  • DNA Fingerprinting / instrumentation*
  • Ethnicity / genetics
  • Female
  • Forensic Sciences / instrumentation*
  • Genetic Loci*
  • Genotype
  • Humans
  • Male
  • Microsatellite Repeats*
  • Polymerase Chain Reaction / methods
  • Saliva
  • Sensitivity and Specificity
  • Sequence Analysis, DNA / methods
  • Species Specificity
  • Y Chromosome*