Roots account for a major part of plant biomass in Tibetan alpine meadows. Understanding root decomposition with global change is key to predict carbon (C) and nutrient dynamics on the Qinghai-Tibet Plateau. Yet, few experiments have carefully examined root decomposition as influenced by global change. We conducted a field study to investigate the effects of nitrogen (N) addition, air warming, precipitation change, and the presence/absence of living roots on root decomposition in a Tibetan alpine meadow. Our results showed that N addition increased the mass and C remaining, and induced N accumulation in the litter. Increased precipitation significantly amplified the positive effect of N addition on litter mass remaining. The presence of alive roots in the litterbags decreased root litter C remaining but significantly increased N and phosphorus remaining of the litter. However, we did not find any significant effects of air warming on the litter decomposition. In the Qinghai-Tibet Plateau, N deposition is predicted to increase and precipitation regime is predicted to change. Our results suggest that the interaction between increased N and precipitation may reduce root decomposition in the Qinghai-Tibet Plateau in the future, and that the large stock of living roots exert a dominant impact on nutrient dynamics of root decomposition in the Tibetan alpine systems.