Photodynamic therapy inhibits cell growth and enhances the histone deacetylase-mediated viability impairment in Cryptococcus spp. in vitro

José Athayde Vasconcelos Morais; Mosar C Rodrigues; Fernanda F Ferreira; Kunal Ranjan; Ricardo Bentes Azevedo; Marcio J Poças-Fonseca; Luis A Muehlmann

doi:10.1016/j.pdpdt.2019.101583

Photodynamic therapy inhibits cell growth and enhances the histone deacetylase-mediated viability impairment in Cryptococcus spp. in vitro

Photodiagnosis Photodyn Ther. 2020 Mar:29:101583. doi: 10.1016/j.pdpdt.2019.101583. Epub 2019 Nov 12.

Authors

José Athayde Vasconcelos Morais¹, Mosar C Rodrigues¹, Fernanda F Ferreira², Kunal Ranjan², Ricardo Bentes Azevedo³, Marcio J Poças-Fonseca², Luis A Muehlmann⁴

Affiliations

¹ Laboratory of Nanoscience and Immunology, Faculty of Ceilandia, University of Brasilia, Brasilia, DF, Brazil; Laboratory of Nanobiotechnology, Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasilia, DF, Brazil.
² Laboratory of Gene Regulation and Mutagenesis, Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasilia, DF, Brazil.
³ Laboratory of Nanobiotechnology, Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasilia, DF, Brazil.
⁴ Laboratory of Nanoscience and Immunology, Faculty of Ceilandia, University of Brasilia, Brasilia, DF, Brazil; Laboratory of Nanobiotechnology, Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasilia, DF, Brazil. Electronic address: [email protected].

PMID: 31731064
DOI: 10.1016/j.pdpdt.2019.101583

Abstract

Cryptococcosis is a disseminated infection caused mainly by C. neoformans and C. gattii. Limitations for the treatment involve the selection of isolates resistant to conventional antifungal drugs, prolonged treatment time and drugs side effects. This study evaluated the combined effect of histone deacetylase inhibitors (HDACi) and photodynamic therapy (PDT) on the growth of C. neoformans and C. gattii in vitro. Results showed that PDT inhibited yeasts proliferation and enhanced the HDACi-mediated cell viability impairment in Cryptococcus spp.

Keywords: Aluminum-phthalocyanine; Cryptococcus spp.; Epigenetics; Photodynamic therapy; Sodium butyrate; Trichostatin A.

MeSH terms

Cell Cycle / drug effects
Cryptococcus / drug effects*
Histone Deacetylase Inhibitors / pharmacology*
Humans
Indoles / pharmacology*
Organometallic Compounds / pharmacology*
Photochemotherapy / methods*
Photosensitizing Agents / pharmacology*

Substances

Histone Deacetylase Inhibitors
Indoles
Organometallic Compounds
Photosensitizing Agents
aluminum phthalocyanine