Generation of two iPSC lines, (ICGi015-A and ICGi015-B), by reprogramming peripheral blood mononuclear cells from a patient with Parkinson's disease

D V Sharipova; V R Kovalenko; E M Bairamova; V A Vartanova; E V Grigor'eva; Y V Vyatkin; E A Khabarova; D A Rzaev; S M Zakian; S P Medvedev

doi:10.1016/j.scr.2019.101652

Generation of two iPSC lines, (ICGi015-A and ICGi015-B), by reprogramming peripheral blood mononuclear cells from a patient with Parkinson's disease

Stem Cell Res. 2019 Dec:41:101652. doi: 10.1016/j.scr.2019.101652. Epub 2019 Nov 9.

Authors

Affiliations

¹ Federal Research Center Institute of Cytology and Genetics, the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia; E.N. Meshalkin National Medical Research Center, Ministry of Health of the Russian Federation, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, Russia.
² Federal Research Center Institute of Cytology and Genetics, the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
³ Federal Research Center Institute of Cytology and Genetics, the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, Russia.
⁴ Novosibirsk State University, Novosibirsk, Russia; AcademGene LLC, Novosibirsk, Russia; St. Laurent Institute, Woburn, USA.
⁵ Federal Neurosurgical Center, Novosibirsk, Russia.
⁶ Federal Research Center Institute of Cytology and Genetics, the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia; E.N. Meshalkin National Medical Research Center, Ministry of Health of the Russian Federation, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, Russia. Electronic address: [email protected].

PMID: 31733442
DOI: 10.1016/j.scr.2019.101652

Abstract

Studying Parkinson's disease (PD), one of the most common neurodegenerative disorders worldwide, requires different model systems, including patient-specific induced pluripotent stem cell lines. With the help of non-integrating episomal vectors the iPSC lines ICGi015-A and ICGi015-B were generated from blood mononuclear cells of PD patient, carrying three SNPs, associated with PD development. The obtained iPSC lines express pluripotency markers and demonstrate the ability to in vitro differentiate into the three germ layers. These cell lines may be useful for studying molecular mechanisms of PD and for drug screening.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Culture Techniques / methods*
Cell Line / pathology*
Cellular Reprogramming*
Female
Humans
Induced Pluripotent Stem Cells / pathology*
Leukocytes, Mononuclear / pathology*
Middle Aged
Parkinson Disease / blood*
Parkinson Disease / pathology*