Objective: To evaluate the value of Gd-BOPTA enhanced MRI in the staging of liver fibrosis. Method: Fifty male SD rats (6-week-old, 180-220 g) were divided into the modeling group (n=42) and the control group (n=8). The model of liver fibrosis in the modeling group was established by carbon tetrachloride (animal license No. SYXK (Su) 2017-0043). From week 2 to week 10, rats in the modeling group (n=4) and control group (n=1) were randomly selected to scan 1 h(RER1), 2 h(RER2) and 3 h(RER3) after injection of Gd-BOPTA, respectively, to measure the relative enhancement rate (RER) of liver parenchyma. The shape of intrahepatic bile duct and the degree of enhancement at each time point were observed. Results: Forty-two rats (34 rats in the modeling group and 8 rats in the control group) completed the experiment. RER1, RER2 and RER3 of the control group were 1.44±0.37, 1.22±0.37 and 0.84±0.28 respectively. RER1, RER2 and RER3 of the modeling group were respectively: S1 (n=6): 1.49±0.48, 1.29±0.39, 0.91±0.38;S2 (n=9): 1.48±0.44, 1.34±0.37, 1.04±0.40;S3 (n=11): 1.49±0.43, 1.37±0.39, 1.21±0.30; S4 (n=8): 1.49±0.44, 1.40±0.37, 1.24±0.40. There was no significant difference in RER1 and RER2 values between the control group and the liver fibrosis group (F=0.022, P=0.999; F=0.301, P=0.875). There were significant differences between the control group and RER3 values of hepatic fibrosis stage S3 and S4 (t=2.249, P=0.031; t=2.274, P=0.029), there was no significant difference between the remaining groups (all P>0.05).In the control group, the intrahepatic bile duct was obviously strengthened within 1 hour after enhancement, and walked naturally. The intrahepatic bile duct was slightly enhanced 1h after the enhancement of S3-S4 stage of hepatic fibrosis, and the intrahepatic bile duct was significantly enhanced 2-3 hours later, with distorted alignment. Conclusion: Delayed 3 hours liver parenchymal RER and intrahepatic bile duct distortion delay enhancement after Gd-BOPTA enhancement contribute to the S3-S4 diagnosis of liver fibrosis.
目的： 探讨钆贝葡胺（Gd-BOPTA）增强磁共振成像对大鼠肝纤维化分期的诊断价值。 方法： 50只雄性SD大鼠（6周龄，体质量180~220 g）分为建模组（n=42）和对照组（n=8），建模组采用四氯化碳法建立肝纤维化模型，动物许可证编号SYXK（苏）2017-0043。实验第2周至第10周每周随机抽取建模组（n=4）和对照组（n=1）大鼠于注射Gd-BOPTA后1 h(RER1)、2 h(RER2)、3 h(RER3）分别扫描，测算肝实质的相对强化率（RER）。观察肝内胆管形态及各时间点强化程度。 结果： 42只大鼠（建模组34只、对照组8只）完成实验。对照组RER1、RER2、RER3分别为1.44±0.37、1.22±0.37、0.84±0.28。建模组RER1、RER2、RER3分别为：S1期（n=6）：1.49±0.48、1.29±0.39、0.91±0.38；S2期（n=9）：1.48±0.44、1.34±0.37、1.04±0.40；S3期（n=11）：1.49±0.43、1.37±0.39、1.21±0.30；S4期（n=8）：1.49±0.44、1.40±0.37、1.24±0.40。对照组与肝纤维化组RER1、RER2值比较差异均无统计学意义（F=0.022，P=0.999；F=0.301，P=0.875）；对照组与肝纤维化S3期、S4期RER3值比较差异有统计学意义（t=2.249，P=0.031；t=2.274，P=0.029），其余各组间比较差异无统计学意义（均P>0.05）。对照组增强后1 h肝内胆管明显强化，走行自然；肝纤维化S3-S4期增强后1 h肝内胆管轻度强化，2~3 h肝内胆管明显强化，走行扭曲。 结论： Gd-BOPTA增强后延迟3 h肝实质相对强化率及肝内胆管扭曲延迟强化有助于肝纤维化S3-S4期诊断。.
Keywords: Diagnosis; Gadolinium; Liver cirrhosis; Magnetic resonance imaging; Rats.